This collection was essentially started with the founding of the museum in 1908 and focus on household appliances. It features a number of kitchen machines and household appliances (refrigerators, washing machines, irons, stoves, electric kettles, blenders, mixers and a wide range of hygienic appliances. The collection contains some 2,000 active inventory numbers.
A specific subset of the collection consists of the Náprstek collection that was transferred to the NTM in 1943 from the former Czech Industrial Museum. Originally the collection was created and developed by Czech progressive Vojta Náprstek (1826-1894) in 1860 – 1890. Following his exile to the USA following 1848 he was captivated by the many interesting technological novelties and household objects that alleviated the work of housewives. He began to buy them, intending to show them back home in Prague. Very much contributing to Náprstek’s purchases were Czech clubs in America. Upon his return home, V. Náprstek continued to expand his collection. He travelled European capitals (London, Vienna, Hamburg, Paris) in updating his collection of technical appliances. After it was transferred to the NTM, the collection was divided into different sections. The group of objects received into the Household Technology Collection has 750 active inventory numbers.

The basis of the collection of Textile production was formed by the object obtained from the textile pavilion of the special anniversary exhibition of the Commerce and Enterprise Chamber in 1908. As the collection grew, it was divided into the following parts: natural and synthetic raw materials, spinning devices and machine, manual and mechanical looms, manual weaving, the colouring and printing of fabrics, sewing machines and production of pins and needles, textiles patterns, pattern books. The development of the textile collection slowed after World War II and today only contains a few samples of the rationalization process of textile production (e.g. in many ways the break-through air jet loom P 45 from 1955). In 2002, the collection was badly damaged by flooding.
Presently the collection is divided into two main parts – textile technology with pattern books and sewing technology. The collection has 960 active inventory numbers.