The printing collection was created in two places up to World War II. The older part comes from the collection of the Czech Industrial Museum founded by Vojtěch Náprstek n which the graphic collection were created beginning in the 1870s; the second part constitute the original collections of the Technical Museum, which was founded in 1911. Both collections were definitively merged in 1944. The collection is immense. There are in total nearly 5,000 items in which some are even broad groups of presses or formes. Some 200 machines form the basis of the collection. These are printing machines, finishing machines, machines for preparing formes and a small section of office machines. There is also a very large collection of formes for all technology, but above all printing blocks. Final products are also presented – books, magazines, posters and other printing material. The collection is divided according to the technological process of print production and according to the various printing techniques: letterpress, intaglio printing (gravure, heliogravure and the collection of relics of Karel Václav Klíč, offset printing (and lithography). Another part is the preparation of formes, foundry and bookbinding finishing work. Each section feature a representative sample of production machines, tools and final products. The wooden letterpress from the 17th century is a standout piece of the collection. Other interesting objects included the first rotary letterpress in the Czech lands, a letterpress from 1877 and a developmental series of lithographic machines.

The collection of writing technology consists of 400 typewriters of roughly a hundred different brands. The most highly represented serial-produced typewriters are of American origin, followed by German, English (Salter, Lloyd), Czech (Zeta, Consul) and there are even Swiss, Norwegian and other production samples. The collection of writing technology is then supplemented by exhibited items documenting the production and use of pencils, pens and several replicas of ancient writing utensils. A collection highlight is the German Westphalia typewriter or the miniature Taurus Type typewriter, which at first glance looks like a pocket watch. The collection also features several typewriters of Czech origin, such as a prototype of the Czech Matouš typewriter, and J. Novák’s curious wooden Aerotype machine and especially an original typewriter, manufactured by Jindřich Odkolek in one specimen.